AM Variant Cell Free gDNA

 

https://anchormolecular.com/

AM Multiplexed ctDNA
Description: DNA contains multiplexed 20+ cell-derived variants each at 5% Allele Frequency. DNA is extracted and fragmented from mutated PAM3005 cells

(Cell Free gDNA)

     Dissolved in TE: Part Number: 60135001
     10ug per vial
     Spiked in Plasma: Part Number: 60135501
     Pre-spiked with 250ng gDNA per 5ml

Variants included

APC Q1429*	KIT D816V
APC R1450*	KRAS G12C
BRAF V600E	KRAS G12D
CTNNB1 T41A	KRAS G12V
EGFR C797S	NRAS Q61R
EGFR E746-A750del	PIK3CA E545K
EGFR L858R	PIK3CA H1047R
EGFR D770_N771insG	PTEN R130G
EGFR T790M	TP53 R175H
FGFR3 S249C	TP53 R248Q
IDH1 R132H

AM Trisomy cfgDNA
Description: DNA is extracted and fragmented from cells harboring trisomy 13, 18 or 21. It is used for PCR- or counting-based NIPT assay
      Dissolved in TE: Part Numbers: 60134001 (Trisomy13), 60134002 (Trisomy18), 60134003 (Trisomy21)
     10ug per vial
     Spiked in Plasma: Part Numbers: 60134501 (Trisomy13), 60134502 (Trisomy18), 60134503 (Trisomy21)
     Pre-spiked with 250ng gDNA per 5ml

(Cell Free gDNA)

Questions?
Our team of scientists is here to help!
Contact Us at info@anchormolecular.com

AM Normal Cell Free gDNA

 

(Cell Free gDNA)

AM Normal cfgDNA-1
Description: DNA is extracted and fragmented from a popular normal male cell line

Dissolved in TE: Part Number: 60133001
10ug per vial
Spiked in Plasma: Part Number: 60133501
Pre-spiked with 250ng gDNA per 5ml

AM Normal cfgDNA-2
Description: DNA is extracted and fragmented from normal female cell line, PAM3005
Dissolved in TE: Part Number: 60133002
10ug per vial
Spiked in Plasma: Part Number: 60133502
Pre-spiked with 250ng gDNA per 5ml

AM Normal cfgDN(Cell Free gDNA)A-3
Description: DNA is extracted and fragmented from pooled normal peripheral blood mononuclear cells (PBMC) from fresh blood
Dissolved in TE: Part Number: 60133003
10ug per vial
Spiked in Plasma: Part Number: 60133503
Pre-spiked with 250ng gDNA per 5ml

(Cell Free gDNA)

Questions?
Our team of scientists is here to help!
Contact Us at info@anchormolecular.com

Anchor SARS-COV-2 Quality Control (Cell Free gDNA)

(Cell Free gDNA)

 Anchor SARS-COV-2 Quality Control

Part Number: 60230001
6 Vials, 2 mL Fill Volume, -20°C Storage
Product Insert
Safety Data Sheet

LIMITATIONS:
For Research Use Only. Not for use in diagnostic procedures.

INTENDED USE:
The Anchor SARS-COV-2 Quality Controls are intended for use by clinical labs and assay manufacturers to assess the performance of any molecular-based testing of SARS-COV-2.

PRODUCT DESCRIPTION:
The Anchor SARS-COV-2 Quality Controls contain a non-pathogenic recombinant RNA virus. This allows for the monitoring of the entire testing process, from the extraction of viral RNA to the end result of the nucleic acid amplification. The virus carries known target sequences (Wuhan-Hu-1, NC_045512.2) used in more than 160 molecular tests worldwide, including the coding regions for ORF1ab, RDRP, S, E, N, M, ORF8 and unique regions among the coronavirus family. For the control of nucleic acids derived from human cells, this virus also carries a 65-base human RNase P sequence used by the Centers for Disease Control and Prevention (CDC) in the 2019-nCoV Real-time RT-PCR. The target titer of the Anchor SARS-COV-2 Quality Controls is 10,000 copies/ml. For the exact value of a specific lot, please refer to the Certificate of Analysis (CoA).(Cell Free gDNA)

(Cell Free gDNA)

Anchor SARS-COV-2 Reference Panel (Cell Free gDNA)

 

(Cell Free gDNA)

Anchor SARS-COV-2 Reference Panel
Part Number: 60230002
4 Vials of positives and 1 vial of negative
(0, 1000, 10,000, 100,000 and 1,000,000 copies/ml,
Negative has 10,000 copy/ml human gDNA),
1 mL Fill Volume, -20°C Storage

Anchor SARS-COV-2 Reference Standard
Part Number: 60230003
1 Vials (500,000 copies/ml)
1 mL Fill Volume, -20°C Storage

Anchor SARS-COV-2 Negative
Part Number: 60230004
1 Vials (10,000 copy/ml human gDNA)
1 mL Fill Volume, -20°C Storage

Product Insert Reference Panel
Product Insert Reference Standard
Safety Data Sheet

LIMITATIONS:
For Research Use Only. Not for use in diagnostic procedures.

(Cell Free gDNA)

INTENDED USE:
The Anchor SARS-COV-2 Reference Panels are intended for use by clinical labs and assay manufacturers to establish a quantitative molecular-based assay for SARS-COV-2 and to validate the assay’s performance.

PRODUCT DESCRIPTION:
The Anchor SARS-COV-2 Reference Standard and Panel are made from a non-pathogenic recombinant RNA virus. The virus carries known target sequences (Wuhan-Hu-1, NC_045512.2) used in more than 160 molecular tests worldwide, including the coding regions for ORF1ab, RDRP, S, E, N, M, ORF8 and unique regions among the coronavirus family. The target titers for panel members are 1,000, 10,000, 100,000 and 1,000,000 copies/ml. The target titer for Reference Standard is 500,000 copy/ml. For the exact value of a specific lot, please refer to the Certificate of Analysis (CoA). 

(Cell Free gDNA)

(Cell Free gDNA)

cfDNA Extraction Sensitivity Controls (Cell Free gDNA)

 

(Cell Free gDNA)

Does your cfDNA extraction process affect the Limit of Detection (LOD) of a particular gene target? Our new products, cfDNA Extraction Sensitivity Panels and Extraction Low Positive Controls, can help your research and development for liquid biopsy assays.

(Cell Free gDNA)

NEW PRODUCTS: cfDNA EXTRACTION SENSITIVITY PANELS &  CONTROLS

The Extraction Sensitivity Controls can help by monitoring plasma cfDNA loss, determining how low the absolute plasma cfDNA number of copies can be reliably extracted, and standardizing QC workflow for plasma cfDNA extraction process.

Two extraction sensitivity panels and two extraction low positive controls are currently available as catalog products. Also, the customized panels and controls can be built per requirements of customer. For the price and availability of catalog and customized products, please send an email inquiry to info@anchormolecular.com.

Extraction Sensitivity Panel 1 (for absolute copies/ml)

• DNA-free plasma spiked with a 170bp specific DNA fragment at 0, 12, 25, 50, 100, 200 copies/ml in a constant 5 ng/ml fragmented gDNA background. Assay for this specific fragment is separately sold.

Extraction Sensitivity Panel 2 (for gDNA pg/ml)

• DNA-free plasma spiked with ~170bp gDNA fragments at 0, 30, 100, 300, 1,000, 3,000 pg/ml. Assay for a house keeping gene is separately sold.

Extraction Low Positive Controls

• DNA-free plasma spiked with ~170bp specific fragments or gDNA at a fixed low concentration.

(Cell Free gDNA)

(Cell Free gDNA)

Application Example for qPCR (Cat. No. 60100040)

(Cell Free gDNA)

(Cell Free gDNA)

Native or synthetic ctDNA fragment can be spiked in a series of concentration in a constant gDNA background. The extraction sensitivity of a synthetic DNA fragment was compared between two cfDNA extraction kits via qPCR. A left graph shows the difference in the ability of extraction at 0 – 200 copies/ml range by the two kits. A right graph compares the %CV of 6 replicates per level.

DNA-Depleted Plasma (Cell Free gDNA)

 

(Cell Free gDNA)

Build your own controls using your high-positive samples to save time. With our DNA-depleted human plasma (DNA-free plasma) as a matrix, you can produce reference materials specific to your time-sensitive needs while retaining the natural components of the sample.

(Cell Free gDNA)

Prefer to have a knowledgeable lab do the work? Send your plasma to Anchor Molecular and we’ll remove the DNA background for you.

Product Name	Cat. No.	Size
DD Plasma (DNA-Depleted Plasma)	60601001	50, 200, 500, 1000 ml
DD Serum (DNA-Depleted Serum)	60601002	50, 200, 500, 1000 ml

Why Is Plasma Better Than Buffer As Reference Material Matrix?

A plasma matrix is a better representation of a real patient sample because the natural, interfering proteins are retained. This allows for a more realistic process in your quality system.

(Cell Free gDNA)

Equal amounts of DNA fragments were spiked into TE Buffer (blue) and human plasma (red).

While the 170 bp peak was recovered similarly in each matrix, much more genomic DNA was extracted from the plasma than from the TE buffer resulting in a more patient-like matrix.
See Figure A.

 

Exosome counts in DNA-depleted plasma are normal compared to regular plasma.

(Cell Free gDNA)

 

AM DNA-Depleted Plasma

• Highest quality DNA-depleted human plasma
• For use in research, assay development, & daily assays
• Retains naturally-occurring, interfering proteins
• Allows for more realistic process in quality systems
• Customized plasma available upon request
• Price upon request

(Cell Free gDNA)

CHEMICAL PRODUCT CHARACTERISTICS

Concentration of DNA:  < 10 pg/mL

Total protein: 5-7 g/dL

Immunoglobulin G: 500-1700 mg/dL

Immunoglobulin A: 90-400 mg/dL

Immunoglobulin M: 20-172 mg/dL

Cholesterol, Total:  50-199 mg/dL

Triglycerides: 50-149 mg/dL

HDL Cholesterol:  > 20 mg/dL

VLDL Cholesterol: 10-40 mg/dL

LDL Cholesterol:  20-99 mg/dL

pH: 7.2-7.6

Tested Negative for HIV, HBV, HCV,

HTLV and Syphilis by FDA approved methods